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KMID : 0903520070500040262
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
2007 Volume.50 No. 4 p.262 ~ p.267
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Isolation of N-lauroyl Tyrosine Antibiotic in E. coli Carrying N-acyl Amino Acid Synthase Gene from Environmental DNA in Korean Soils
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Yeo Yun-Soo
Lim Yoong-Ho
Kim Jung-Bong
Yang Jung-Mo
Lee Chang-Muk
Kim Soo-Jin
Park Min-Seon
Koo Bon-Sung
Yoon Sang-Hong
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Abstract
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To access the natural product antibiotics produced by uncultured microorganisms, six cosmid libraries of DNA extracted directly from soil samples (environmental DNA, eDNA) were con- structed and screened for the production of antibacterial active molecules. Of the approximately 60,000 clones screened, one antibacterial clone (YS92B) was detected. Ethyl acetate extracts of clone YS92B showed antibacterial activity against various pathogenic bacteria (Listeria monocyto- genes, Bacillus subtilis, Pseudomonas syringae, Xanthomonas campestris pv. oryzae, Staphylococcus epidemis). Active constituents from cultures of YS92B were isolated and purified using a bioassay- guided fractionation against B. subtilis through a series of procedures (ethyl acetate extraction, Sephadex LH20 column chromatography, High Performance Liquid Chromatography). NMR (Nuclear Magnetic Resonance) spectral analysis of a major antibacterial active YS92B-VII indi- cated that it is a lauric acid linked to tyrosine. This report describes the characterization of anti- bacterially active long chain N-acyl derivatives of tyrosine that are produced by eDNA clones hosted in Escherichia coli from Korean soils.
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KEYWORD
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eDNA, metagenomic library, N-acyl tyrosine antibiotics
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